Info • Laccaria sp. KIS1 v1.0

Status

[February 2025] The Laccaria sp. KIS1 v1.0 genome was sequenced with PacBio, assembled with Flye, and annotated with the JGI Annotation Pipeline. The transcriptome was sequenced with Illumina and assembled with Trinity.

This genome is likely a polymorphic dikaryon, and this is reflected in an assembly and annotation with significant separation of alleles. Some of the scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertoire of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.

Genome Assembly
Genome Assembly size (Mbp) 231.43
Sequencing read coverage depth 75.02x
# of contigs 1755
# of scaffolds 1755
# of scaffolds >= 2Kbp 1755
Scaffold N50 327
Scaffold L50 (Mbp) 0.21
# of gaps 0
% of scaffold length in gaps 0.0%
Three largest Scaffolds (Mbp) 1.85, 1.64, 1.47


The transcriptome suffered from significant bacterial contamination, leading to poor genome-mapping rates.
ESTs Data set # sequences total # mapped to genome % mapped to genome
EstClusters ESTclusters 204983 116120 56.6%
Ests est.fasta 179570625 151725753 84.5%


Gene Models FilteredModels1
length (bp) of: average median
gene 1567 1303
transcript 1221 975
exon 233 142
intron 83 56
description:
protein length (aa) 368 284
exons per gene 5.25 4
# of gene models 40277


Funding

The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.